Product Details
Neomycin sulfate belongs to the aminoglycoside antibiotic family and also acts as a calcium channel protein inhibitor. It attaches to prokaryotic ribosomes to block bacterial protein translation, showing activity against both gram-positive and gram-negative bacteria.
It suppresses Phospholipase C (PLC) by binding to phospholipids. Besides, it inhibits phosphatidylcholine-PLD activity, triggers Ca²⁺ mobilization and activates PLA2 in human platelets.
Moreover, it can prevent DNase I-mediated DNA degradation.
This product is applied for the prevention and treatment of bacterial skin infections. It has no effect on fungal or viral infections.
Chemical Properties
| Melting point | >187°C (dec.) |
| alpha | D20 +54° (c = 2 in H2O) |
| bulk density | 640kg/m3 |
| refractive index | 56 ° (C=10, H2O) |
| Fp | 56℃ |
| storage temp. | 2-8°C |
| solubility | H2O: 50 mg/mL As a stock solution. Stock solutions should be filter sterilized and stored at 2-8°C. Stable at 37°C for 5 days. |
| form | powder |
| color | white to slightly yellow |
| PH | 5.0-7.5 (50g/l, H2O, 20℃) |
| biological source | Streptomyces sp. |
| Water Solubility | Soluble in water |
| Merck | 14,6454 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| InChIKey | OIXVKQDWLFHVGR-VTSVPHRWSA-N |
| LogP | -3.655 (est) |
| CAS DataBase Reference | 1405-10-3(CAS DataBase Reference) |
| EPA Substance Registry System | Neomycin sulfate (1405-10-3) |
Safety Information
| Hazard Codes | Xn,Xi |
| Risk Statements | 42/43-22 |
| Safety Statements | 23-36/37-45-22-36-24/25 |
| WGK Germany | 3 |
| RTECS | QP4375000 |
| F | 8 |
| TSCA | Yes |
| HS Code | 29419000 |
| Toxicity | LD50 oral in mouse: > 8gm |
Product Usage
Produced by Streptomyces fradiae, neomycin sulfate is categorized as an aminoglycoside antibiotic. It inhibits protein translation via binding to the small subunit of prokaryotic ribosomes.
It functions to block voltage‑sensitive Ca²⁺ channels and acts as a powerful inhibitor of Ca²⁺ release from the sarcoplasmic reticulum in skeletal muscle.
It has demonstrated inhibitory effects on inositol phospholipid turnover, phospholipase C, and phosphatidylcholine‑phospholipase D activity, with an IC₅₀ value of 65 μM.
It possesses potent bactericidal activity toward Gram‑positive and Gram‑negative bacteria, and is routinely applied for the prophylaxis of bacterial contamination in cell culture work.
