Methylene Blue Chemical Properties
Melting point | 190 °C (dec.)(lit.) |
density | 1.0 g/mL at 20 °C |
refractive index | n20/D1.347 |
Fp | 45 °C |
storage temp. | room temp |
solubility | Soluble in water, ethanol, ethylene glycol, methyl cellosolve |
form | Liquid |
pka | 2.6, 11.2(at 25℃) |
Colour Index | 52015 |
color | Green |
Specific Gravity | 0.98 |
Odor | Odorless |
Water Solubility | 40 g/L (20 ºC) |
λmax | 661 nm |
Merck | 14,6060 |
BRN | 3641570 |
Biological Applications | Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases |
NIST Chemistry Reference | Methylene blue(61-73-4) |
EPA Substance Registry System | Methylene blue (61-73-4) |
Safety Information
Hazard Codes | Xn |
Risk Statements | 10-41-22-20/21/22-36/37/38 |
Safety Statements | 26-39-36 |
RIDADR | UN 1993 3/PG 3 |
WGK Germany | 3 |
RTECS | SP5740000 |
HS Code | 32049010 |
Hazardous Substances Data | 61-73-4(Hazardous Substances Data) |
Toxicity | LD50 oral in rat: 1180mg/kg |
Product Overview
Methylene Blue, also known as Methylthioninium Chloride, is a synthetic thiazine dye identified by CAS number 61-73-4. It is characterized by its deep blue color and is widely recognized in laboratory staining, microscopy and dye-related applications.

Safety and Storage
Methylene Blue should be handled according to the product-specific Safety Data Sheet (SDS). Avoid inhaling dust and prevent direct contact with eyes, skin and clothing. Use suitable gloves, eye protection and adequate ventilation.
Store in a tightly sealed container in a cool, dry place, protected from excessive heat and direct light. Keep away from incompatible materials and follow the supplier's storage recommendations.