METHYLENE BLUE

1. Distinctive Blue Color: A well-known blue thiazine dye with strong visible coloration.

2. Water Solubility: The chloride salt is soluble in water, supporting aqueous preparation.

3. Staining Properties: Commonly used in biological staining and microscopy.

4. Laboratory Applications: Suitable for selected analytical and educational laboratory uses.

5. Versatile Dye: Used in various dyeing and coloration applications.

6. Recognized Chemical Identity: Also known as Basic Blue 9 and C.I. 52015.

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Methylene Blue Chemical Properties

Melting point 

190 °C (dec.)(lit.)

density 

1.0 g/mL at 20 °C

refractive index 

n20/D1.347

Fp 

45 °C

storage temp. 

room temp

solubility 

Soluble in water, ethanol, ethylene glycol, methyl cellosolve

form 

Liquid

pka

2.6, 11.2(at 25℃)

Colour Index 

52015

color 

Green

Specific Gravity

0.98

Odor

Odorless

Water Solubility 

40 g/L (20 ºC)

λmax

661 nm

Merck 

14,6060

BRN 

3641570

Biological Applications

Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases

NIST Chemistry Reference

Methylene blue(61-73-4)

EPA Substance Registry System

Methylene blue (61-73-4)

Safety Information

Hazard Codes 

Xn

Risk Statements 

10-41-22-20/21/22-36/37/38

Safety Statements 

26-39-36

RIDADR 

UN 1993 3/PG 3

WGK Germany 

3

RTECS 

SP5740000

HS Code 

32049010

Hazardous Substances Data

61-73-4(Hazardous Substances Data)

Toxicity

LD50 oral in rat: 1180mg/kg

Product Overview

Methylene Blue, also known as Methylthioninium Chloride, is a synthetic thiazine dye identified by CAS number 61-73-4. It is characterized by its deep blue color and is widely recognized in laboratory staining, microscopy and dye-related applications.

METHYLENE BLUE CAS#61-73-4

Safety and Storage

Methylene Blue should be handled according to the product-specific Safety Data Sheet (SDS). Avoid inhaling dust and prevent direct contact with eyes, skin and clothing. Use suitable gloves, eye protection and adequate ventilation.

Store in a tightly sealed container in a cool, dry place, protected from excessive heat and direct light. Keep away from incompatible materials and follow the supplier's storage recommendations.

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