BASIC BLUE 9

1. Distinctive Blue Color: Provides a characteristic deep blue coloration.

2. Water Solubility: The chloride salt is soluble in water.

3. Staining Properties: Widely recognized for biological staining applications.

4. Laboratory Use: Suitable for selected microscopy and analytical applications.

5. Dye Versatility: Used in various coloration and dyeing processes.

6. Multiple Industry Applications: Relevant to laboratory, educational and industrial dye applications.

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BASIC BLUE 9 Chemical Properties

Melting point 

190 °C (dec.)(lit.)

density 

1.0 g/mL at 20 °C

refractive index 

n20/D1.347

Fp 

45 °C

storage temp. 

room temp

solubility 

Soluble in water, ethanol, ethylene glycol, methyl cellosolve

form 

Liquid

pka

2.6, 11.2(at 25℃)

Colour Index 

52015

color 

Green

Specific Gravity

0.98

Odor

Odorless

Water Solubility 

40 g/L (20 ºC)

λmax

661 nm

Merck 

14,6060

BRN 

3641570

Biological Applications

Detecting microorganisms; treating diabetic retinopathy,macular degeneration,malignant uveal melanomas,erysipelas,hidradenitis suppurativa,inflammation,skin diseases

NIST Chemistry Reference

Methylene blue(61-73-4)

EPA Substance Registry System

Methylene blue (61-73-4)

Safety Information

Hazard Codes 

Xn

Risk Statements 

10-41-22-20/21/22-36/37/38

Safety Statements 

26-39-36

RIDADR 

UN 1993 3/PG 3

WGK Germany 

3

RTECS 

SP5740000

HS Code 

32049010

Hazardous Substances Data

61-73-4(Hazardous Substances Data)

Toxicity

LD50 oral in rat: 1180mg/kg

Product Description

Basic Blue 9, commonly known as Methylene Blue or Methylthioninium Chloride, is a synthetic cationic thiazine dye with CAS number 61-73-4. It is characterized by its distinctive deep blue color and is widely used in biological staining, microscopy, laboratory research and dye-related applications.

Main Applications

- Biological Staining: Used for staining selected biological specimens and cellular structures.

- Microscopy: Applied in laboratory microscopy and educational demonstrations.

- Microbiology: Used in selected staining procedures for observing microorganisms.

- Analytical Chemistry: Used as a colorimetric or redox-related reagent in certain analytical methods.

- Industrial Dyeing: Applied as a blue colorant in certain textile, paper and other dye formulations.

- Laboratory Research: Used in scientific research and teaching environments.

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